Cancer stem cells Glioblastoma Multidrugs resistance Adenosine and receptors Resistance mechanisms Clinical studies in cancer Cancer treatment alternatives Angiogenesis

Human Capital Formation

Undergraduate Sponsorship (23)

-2022 Barrientos D. “Role of the A2B adenosine receptor in the maintenance of the stem phenotype of glioblastoma stem-like cells of the mesenchymal subtype and determination of the content of markers associated with stemness of their EVs under hypoxic
conditions”. BIOCHEMISTRY. Universidad Austral de Chile. (In progress)
-2022 Fabre K. P “Study of the role of the A3 adenosine receptor on autophagy-mediated therapeutic resistance in subclasses of Glioblastoma Stem-like Cells”. BIOCHEMISTRY. Universidad Austral de Chile. (In progress)
-2022 Uribe D. “Study of the expression of genes related to chemoresistance under the effect of adenosine a3 receptor antagonist in glioblastoma stem-like cells”. BIOCHEMISTRY. Universidad Austral de Chile. (In progress)
-2020 Uribe A. "Role of adenosine deaminase in the invasive phenotype of glioblastoma stem-like cells under hypoxic conditions”. BIOCHEMISTRY. Universidad Austral de Chile.
-2019. Toro MA. "Study of the expression and activity of nucleoside transporters in glioblastoma stem-like cell”. BIOCHEMISTRY. Universidad Austral de Chile.
-2019. Wagner G. "Participation of A2b and A3 adenosine receptors in the regulation of stem characteristics of glioblastoma stem-like cells". MEDICAL TECHNOLOGY. Universidad Austral de Chile
-2018. Delgado J. "Analysis of the PI3K/Akt signaling pathway and its role in low-affinity adenosine receptor-mediated chemoresistance in human glioblastoma multiforme initiator cells”, BIOCHEMISTRY. Universidad Austral de Chile.
-2017. Ríos J. "Evaluation of genes related to chemoresistance and infiltration regulated by the A3 adenosine receptor in glioblastoma stem-like cells”. CHEMISTRY AND PHARMACY. Universidad Andrés Bello
-2017. Ojeda K. '' Role of adenosine on the secretion of VEGF and differentiation of glioblastoma stm-likeCells to vascular endothelial cells under conditions of hypoxia. BIOCHEMISTRY. Universidad Austral de Chile.
-2017. Delgado J. '' Analysis of the PI3K / Akt signaling pathway and its role in drug-mediated chemoresistanceAdenosine low affinity receptors on human Glioblastoma multiforme primers ".BIOCHEMISTRY. Universidad Austral de Chile.
-2017. Erices J. '' Study of the role of adenosine in the invasive capacity of glioblastoma stem-like cells underConditions of hypoxia ". BIOCHEMISTRY. Universidad Austral de Chile.
-2016. Arriagada V. '' Study of the effect of tacrolimus on multiple drug resistance of stem-like cells ofGlioblastoma multiforme human ". BIOCHEMISTRY. Universidad Austral de Chile.
-2015. Vargas Y. Study of multiple drug resistance modulators in an in vivo allogeneic model ofGlioblastoma ". BIOCHEMISTRY. Universidad Austral de Chile.
-2015. Uribe D. Study of the effect of hypoxia on multiple drug resistance in stem-like cells ofGlioblastoma ". BIOCHEMISTRY. Universidad Austral de Chile.
-2015. González C. '' Study of signaling pathways involved in the chemoresistance in GlioblastomaMediated by Adenosine ". BIOCHEMISTRY. Universidad Austral de Chile.
-2014. Dellarossa A. Role of adenosine in multiple drug resistance in glioma-starter cells ofhigh grade''. BIOCHEMISTRY. Universidad Austral de Chile.
-2014. Hueicha K. Effect of MRP1 chemosensitizers on cytotoxicity and antiproliferation of drugsAntitumor agents in human glioblastoma multiforme primer cells ". MEDICAL TECHNOLOGY. Universidad Austral de Chile.
-2014. Rocha J. Study of the MRP1 drug resistance transporter in Gliobastoma multiforme human ". BIOCHEMISTRY. Universidad Austral de Chile.
-2013. Vergara R. '' Characterization of drug resistance transporters in gynecological tumors ''.BIOCHEMISTRY. Universidad Austral de Chile.
-2013. Cid M. Effect of Sulindac on the activity of multiple drug resistance proteins inHuman multiform glioblastomas ". MEDICAL TECHNOLOGY. Universidad Austral de Chile.
-2012. Muñoz M. '' Tacrolimus modulates the expression and activity of drug resistance transporters in Brain tumors ". CHEMISTRY AND PHARMACY. Universidad Austral de Chile.
-2011. Salinas J. Effect of inhibition of the MRP1 transporter on the chemoresistance in glioblastoma Human multiforme ". CHEMISTRY AND PHARMACY. Universidad Austral de Chile.
-2010. Cabezas G. '' Quantification of the expression and determination of the location of the transporters of Drug resistance MRP-1, P-gp and ABCG2 in cell lines and primary culture of brain tumors humans''. CHEMISTRY AND PHARMACY. Universidad Austral de
Chile.
-2010. Peigñan L. 'Evaluation of the inhibition of MRP1 transporter activity on cytotoxicity and Antiproliferative effect of antineoplastic drugs in the T98G cell line and primary culture of glioblastoma Human multiforme ". BIOCHEMISTRY. Universidad Austral de Chile.
-2009. Alarcón S. '' Effect of diabetic syndrome on the expression and activity of ABC proteins ''. BIOCHEMISTRY. Universidad Austral de Chile.
-2008. Garrido W. '' Regulation of the expression and activity of proteins of the ABC family and non ABC by tacrolimus and cyclosporin A in hematopoietic barrier and brain tumor cells. " BIOCHEMISTRY. Universidad Austral de Chile.

Undergraduate Co-Sponsorship (6)

-2015. Rubilar P. The variation of the expression of immunophilin 65 (FKBP65) in the diagnosis and prognosis of colon cancer. Defining a possible early marker ". MEDICAL TECHNOLOGY. Universidad Austral de Chile.
-2014. Bórquez C. '' Estimation of the incidence of genetic polymorphisms associated with the pharmacogenetics of Methotrexate and its clinical implications in pediatric patients with acute lymphoblastic leukemia. " CHEMISTRY AND PHARMACY. Universidad Austral de Chile.
-2014. Ross T. Generation of a plasmid vector for the overexpression of the adenosine A2B receptor in Renal cell lines ". Bachelor of Science in Biological Sciences. Universidad Austral de Chile.
-2014. Vidal Y. Study of the differentiation in glioblastoma-initiating cells in the presence of a new Polymer matrix ". CHEMISTRY AND PHARMACY. Universidad Austral de Chile.
-2013. Kretschmar C. '' Role of adenosine receptors on epithelial-mesenchymal transdifferentiation In HK-2 "human proximal tubule cells. BIOCHEMISTRY. Universidad Austral de Chile.
-2013. Cofré A. Effect of tacrolimus on the expression of multiple resistance proteins to MVP andRLIP76 in T lymphocytes and its relation to the secretion of interleukin-2 ". MEDICAL TECHNOLOGY. Universidad Austral de Chile.

Postgraduate Sponsorship (5)

-2022. José Ignacio Erices. "Study of the Hypoxic Microenvironment on the Invasive Phenotype of Glioblastoma Stem-Like Cells". PhD IN SCIENCES, MENTION CELLULAR AND MOLECULAR BIOLOGY. Universidad Austral de Chile.
-2020. Daniel Uribe. "Study of the role of A2B and A3 adenosine receptors in the maintenance of the stem phenotype of glioblastoma stem-like cells". PhD IN SCIENCES, MENTION CELLULAR AND MOLECULAR BIOLOGY. Universidad Austral de Chile
-2019. J. Dellis Rocha . "Role of adenosine in the chemoresistance of stem-like glioblastoma cells under conditions of hypoxia" . PhD IN SCIENCES, MENTION CELLULAR AND MOLECULAR BIOLOGY. Universidad Austral de Chile.
-2017. Angelo Torres. ''Generation of a high-grade brain tumor model in rats. In vivo study of Multiple drug resistance chemosensitizers". PhD IN VETERINARY SCIENCES. Universidad Austral de Chile.
-2013. Wallys Garrido. ''Role of nucleoside adenosine in chemoresistance of human glioblastoma multiforme''. PhD IN SCIENCES, MENTION CELLULAR AND MOLECULAR BIOLOGY. Universidad Austral de Chile.

In progress (4)

-PhD (Candidate) Liuba Peñate, Scholarship CONICYT PhD in Sciences, Cellular and Molecular Biology, Faculty of Sciences, Universidad Austral de Chile (In progress)
-PhD. (Candidate) Arnaldo Rosales, Scholarship CONICYT PhD in Sciences, Cellular and Molecular Biology, Faculty of Sciences, Universidad Austral de Chile (In progress)
-PhD. (Candidate) Carolina Villarreal, Scholarship CONICYT PhD in Sciences, Cellular and Molecular Biology, Faculty of Sciences, Universidad Austral de Chile (In progress)
-PhD. (Candidate) Hilda Alfaro, Scholarship CONICYT PhD in Sciences, Cellular and Molecular Biology, Faculty of Sciences, Universidad Austral de Chile (In progress)
-MsSc. Brenda Águila. Master in Biochemistry Biotechnology. Science Faculty, Universidad Austral de Chile. (In progress)

Co-sponsorship

-Mr. Erwin Budinich Murúa. Magister in Sciences mention Pathology, Faculty of Medicine, Universidad Austral de Chile. Thesis topic: Role of the Kinase B1 Receptor in the Epithelial-Mesenchymal and Mesenchymal-Epithelial Transition of Breast Cancer Cells (2016).

Postdoc (5)
-Sponsor Investigator CONICYT Postdoctoral Projec "Glycosylated delphinidins as a sensitizing compound to temozolomide treatment in glioblastoma cells by regulating NF-?B/MGMT and COX- 2/MDR1 signaling" (No. 3220237). 2022-2025.
-Sponsor Investigator CONICYT Postdoctoral Projec "Papel de adenosina con la capacidad invasiva de las glioblastoma stell-like celIs. Evaluación de una estrategia terapéutica para disminuir la invasión" (No. 3180621). 2018-2021.
-Sponsor Investigator CONICYT Postdoctoral Project. "Characterization of glioblastoma stem cells with Differences in adenosine metabolism. Implications in the phenomenon of multiple drug resistance " (No. 3170851). 2017-2020.
-Sponsor Investigator CONICYT Postdoctoral Project. "Signaling mechanisms involved in the Cell chemoresistance induced by the adenosine A3 receptor on stem-like cells of glioblastoma multiform human". (No. 3150626). 2014-2017.
-Sponsor Investigator CONICYT Postdoctoral Project. "Role of nucleoside adenosine in the ability Angiogenic activity of human glioblastoma multiforme priming cells "(No. 3140217). 2013-2016.

Technological Transfer Projects.

-Sponsor Investigator Stage II Project FONDEF VIU16P0145 "Kit for the detection of markers of stem cells Mesenchymal and proneural in samples of glioblastoma. 2017-2018.
-Sponsor Investigator Stage I Project FONDEF VIU16P0145 "Kit for the detection of markers of stem cells Mesenchymal and proneural in samples of glioblastoma. 2016-2017.
-Researcher FONDEF D08I1096. '' Adaptation and implementation of VLPS production method (virus as Particles) in insect cells and / or larvae for the development of low cost oral vaccines using a model of generation of vaccine against IPNV ''. 2009-2012.
-Project Director FIC17-19 "Biomarkers in Gallbladder Cancer". Project Regional collaborative research funds with the Hospital Base Valdivia and the Millennium Institute of Immunology and Immunotherapy
-Project Director FIC21-19 "Personalized therapy in Gallbladder Cancer". Project Regional collaborative research funds with the Hospital Base Valdivia and the Millennium Institute of Immunology and Immunotherapy

Management Projects

-Responsible Researcher Project VIII MEDIUM SCIENTIFIC TECHNOLOGICAL EQUIPMENT COMPETITION - FONDEQUIP 2019. "Update of the isolation unit and characterization of cellular subpopulations involved in human and animal pathologies". Cytometer equipment with cell sorter FACS Melody brand BD.
-Associate Researcher and Coordinator UACh Headquarters of the Millennium Institute of Immunology and Immunotherapy (IMII).2019-2020
- Principal Investigator of Research agreement "STUDENT INVESTIGATION DEVELOPMENT CENTER", International Internships Agreement UACH-University of Puerto Rico. 2015-2017.
-Investigator Responsible FONDEQUIP. I Medium Technological Scientific Equipment Contest: Unit of isolation and characterization of cellular subpopulations involved in animal and human pathologies. "Cytometer equipment with the FACSJAZZ cell sorter BD brand. .2012-2014.
- FIDECNO researcher 2010-2011. Collaborative project with the Department of Neurological Sciences of the Instituto Ascenjo Neurosurgery INCA. Prevalence of glioblastoma multiforme in Chile and studies of resistance to chemotherapy mediated by ABC transporters. 2010-2011.

Collaborative and student transfer agreements
-2022-to date. Principal Researcher and Coordinator UACh Headquarters of the Millennium Institute of Immunology and Immunotherapy (IMII).
-2019-2021 Associate Researcher and Coordinator UACh Headquarters of the Millennium Institute of Immunology and Immunotherapy (IMII).
-2015. to date. Collaboration Agreement. International Agreement. Participation in the program STUDENT RESEARCH DEVELOPMENT CENTER, Metropolitan University, San Juan, Puerto Rico for Research stays at the Laboratory of Molecular Pathology of the UACh. Responsible Investigator.
-2012. - to date. Collaboration agreement. National Convention. Scientific Research Agreement with the Valdivia Base Hospital, Chile. (Projects FONDECYT 1121121, 1160777, 1200885). Responsible Investigator.
-2012. - to date. Collaboration agreement. National Convention. Scientific Research Agreement with the Institute of Asenjo Neurosurgery, Santiago, Chile. (Projects FONDECYT 1121121, 1160777, 1200885). Responsable investigator
-2012. - to date. Collaboration agreement. International Agreement. Collaboration with the International Cooperation "Red Glial Ibero-America" (RGIA). Coordinator Dr. Bernardo Castellano, Barcelona Spain. Coinvestigator.

Extension and dissemination to the Community
-Participation in Symposium "Recent advances in metabolism and disease". "Adenosine metabolism as a therapeutic target for glioblastoma". Valdivia March 21, 2019.
-Participation and speaker in Seminars Cycle of the Department of Biomedical Sciences of the Universidad Católica del Norte “Glioblastomas and their chemosensitization to antitumor drugs”. Coquimbo October 27, 2018.
-Talk for Doctors Coquimbo Hospital "Study of drug resistance in glioblastoma". Coquimbo October 26, 2018
-Exhibitor 3rd Science Fair College Maria Auxiliadora "Cancer and drug resistance". College Maria Auxiliadora, Valdivia October 2, 2018.
-Talk for Medicos Hospital Puerto Montt "Detection of pro-neural and mesenchymal stem cells markers in glioblastoma samples". Puerto Montt, August 17, 2018.
-Exhibitor 2nd Scientific Cafeteria “El Café de Renata” Science Festival 2018 of Explora by CONICYT Los Ríos. Valdivia. October 5, 2018.
-Participation in the “Let's Open Our Laboratories Program of the PAR Explora region of Los Rios”. Topic: Effect of natural compounds on brain cancer tumor stem cells. Students third half Liceo Bicentenario de Excelencia Altamira de Panguipulli. April to December 2018
-Exhibitor Workshop of cell cultures of cold waterfish and alternatives primary cell culture (from fish to mouse and human’s methods). "Obtaining Stem-like cells from primary cultures of Glioblastoma: studies on chemoresistance and cell invasion". Austral University of Chile, Valdivia. March 2018.
- Exhibitor 1st Scientific Cafeteria “El Café de Renata” Science Festival 2017 of Explora by CONICYT Los Ríos. Valdivia. October 06, 2017.
- Talks for students Chile VA Project. Llifen Chile Boarding School. July 2016
-2017 - to date. Director of Dissemination of Undergraduate Careers of the Faculty of Sciences.Universidad Austral de Chile.
-Conference for Medical Doctors and Medical interns. Institute of Neurosurgery Dr. Asenjo "Role of hypoxia and adenosine in chemoresistance of subtypes of stem cells of Glioblastoma Multiforme: evaluation of personalized therapies". Santiago, April 2016
-Simposio for Biomedicine Association of Students of Biochemistry ANEB Universidad Austral de Chile. September 2015.
-Conferencefor for Medical Doctors and Medical Interns in the Department of Pathology Valdivia Base Hospital. Study of multiple drug resistance in human gliomas: Strategies for the optimization of the pharmacological treatment of brain tumors. Valdivia March 2014.
-Simposium for Cancer Association of Students of Biochemistry ANEB 2014. Pontificia Universidad Católica de Chile and Universidad Andrés Bello, Santiago. November 2014.
-Inaugural Conference Program Explore CONICYT Region de los ríos. "Combating drug resistance in cancer." Aula Magna Universidad Austral de Chile. Valdivia April 2014.
-Conference for school students "How to be a scientist". November 2014, San Luis de Alba College, Valdivia, Chile.
-Conference for school students "How to be a scientist". November 2013, San Luis de Alba College, Valdivia, Chile.
-Charla for Medical Doctors and Medical interns. Institute of Neurosurgery Dr. Asenjo "Latest advances in the study of multiple drug resistance in glioblastoma multiforme", Santiago. December 2012
-Responsable Professor in course "Medicine of future: Manipulate genes to cure diseases" at the Talent School (ALTA-UACH) of Universidad Austral de Chile. 2010.
-Conference "Cancer and Multiple Drug Resistance", Program CONICYT, Dissemination and Evaluation of Science and Technology, XII National Science and Technology Week "1000 scientists 1000 classrooms". October 2008, San Luis de Alba College, Chile.
-Conference "Resistance and Cancer," Program CONICYT Explore, Dissemination and Evaluation of Science and Technology, XII national science and technology week "1000 scientists 1000 classrooms". October 2007, Prince of Asturias College, Valdivia, Chile.
-Participation in National Television program of Chile (TVN) "Hunters of science" 2008. Theme: "Leptocarpina as a possible antitumor agent".

Management of national and international scientific events
-2017. Organization of Scientific Event. National. Organization symposium "Exploring the winding path of cancer". Society of Biochemistry and Microbiology 2017. Puerto Varas 26 to 29 September 2017. Organizer and Responsible for the Scientific Event.
-2016. Organization of Scientific Event. National. Organization Seminar Series of the Institute of Biochemistry. Science Faculty. Coordinator.
-2015. Organization of Scientific Event. International. Organization I Workshop in Molecular Pathology: "Cancer Stem cells biology and nucleoside metabolism in diseases. Responsible investigator.
-2013. Organization of Scientific Event. International. Organization of National Scientific Symposium. "Paper Nucleoside transporters in pathologies ". Collaborator.
-2013. Organization of Scientific Event. International. Organization International Symposium "Cancer and Inflammation: of the joints adherent to the disease ". Collaborator.
-2011. Organization of Scientific Event. National. Organizer Symposium "Role of adenosine in Pathologies". UACh. Responsible investigator.