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Dolores Busso PERKINS

Associate Professor

Universidad de Los Andes

Santiago, Chile

Líneas de Investigación


Our work is aimed at understanding how nutritional imbalances influence metabolism and reproduction.

Educación

  •  PhD in Biology (Physiology), Universidad de Buenos Aires. Argentina, 2005

Experiencia Académica

  •   Assistant Investigator Full Time

    PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

    Santiago, Chile

    2010 - 2012

  •   PostDoc Full Time

    PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

    Faculty of Biological Sciences

    Santiago, Chile

    2005 - 2009

  •   PhD Student Full Time

    Instituto de Biología y Medicina Experimental - Universidad de Buenos Aires

    Buenos Aires, Argentina

    1998 - 2005

  •   Fellow Full Time

    Institut de la Sante et Researche Medicale

    Paris, Francia

    1997 - 1997

  •   Undergraduate Fellow Part Time

    Centro de Educación Médica e Investigaciones Clínicas

    Buenos Aires, Argentina

    1996 - 1996

  •   Assistant Professor Full Time

    PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

    Faculty of Medicine

    Santiago, Chile

    2013 - 2016

  •   Associate Professor Full Time

    PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

    Faculty of Medicine

    Santiago, Chile

    2016 - 2019

  •   Associate Professor Full Time

    Universidad de los Andes

    Faculty of Medicine

    Santiago, Chile

    2019 - A la fecha

Formación de Capital Humano


Undergraduate Research Units

2006-2008. Mentor Biological Sciences student María José Oñate-Alvarado.
2009. Co-Mentor Biological Sciences student María José Marin.
2009. Co-Mentor Biological Sciences student Sebastian Pereda.
2010. Mentor Biochemistry student Nicolás Santander.
2011-2012. Mentor Medicine student Lilian Mascareño.
2012. Mentor Medicine student Isabella Passalaqua.
2012. Mentor Biological Sciences student Linda Quilaqueo.
2014 -2015. Mentor Biochemistry student Paz Molina.
2014 - 2016. Mentor Medicine student Andrés Schneider.
2016. Mentor Medicine student Daniel Gallardo.
2018. Mentor Biochemistry student Maximiliano Villarreal.
2019. Mentor Nutrition students Perina Kutulas and Magdalena Gutiérrez.

Undergraduate thesis

2011-2012. Thesis in Biochemistry. Nicolás Santander.
2013 - 2014. Thesis in Medical Technology. Constanza Andrea Baeza y Nathaly De La Jara
2013 - 2015. Thesis in Biochemistry. Alonso Quiroz.
2016 - 2017. Thesis in Biochemistry. Paz Molina.
2019. Thesis in Medical Technology. José Chavez.
2019. Thesis in Medical Technology. Nicolás Gonzalez.

Mentoring of international students

2011. MISTI-PUC Grant Fellowship. Allison Hamilos, from MIT (US)
2012. PLISSER Fellowship (Programa de Investigaciones en Salud Sexual y Reproductiva, Asociación Latinoamericana de Investigadores en Reproducción Humana). Natalia Pascuali, Universidad de Buenos Aires (Argentina)
2014. PLISSER Fellowship (Programa de Investigaciones en Salud Sexual y Reproductiva, Asociación Latinoamericana de Investigadores en Reproducción Humana). Paula Accialini Universidad de Buenos Aires (Argentina)
2015. MISTI-PUC Grant Fellowship. Dalia Walzer, from MIT (US)

Graduate Students

2012. Research Unit. Ph.D. Programme in Medical Sciences, School of Medicine. María José Parga.
2013. Research Unit. Ph.D. Programme in Medical Sciences, School of Medicine. Nicolás Santander.
2015. Research Unit. Ph.D. Programme in Medical Sciences, School of Medicine. Loni Berlowitz.

Masters Thesis

2012-2013. Co-Mentor. Master in Nutrition. School of Medicine. Grettel Martelo.
2016. Mentor. Master in Nutrition. School of Medicine. Marcela Riquelme.
2017. Mentor. Master in Nutrition. School of Medicine. Andrea David.

Ph.D. Thesis

2013 - 2016. Mentor. Ph.D. Programme in Physiology. School of Biology. Francisca Salas.
2013 - 2016. Co-Mentor. Ph.D. Programme in Medical Sciences, School of Medicine. Susana Contreras.
2014 - 2018. Mentor. Ph.D. Programme in Medical Sciences, School of Medicine. Nicolás Santander.
2017 - present. Mentor. Ph.D. Programme in Medical Sciences, School of Medicine. Alonso Quiroz.
2019 - present. Mentor. Ph.D. Programme in Biology (Physiology). School of Biology. Andreina Arias.


Difusión y Transferencia


MAIN OUTREACH ACTIVITIES

2007-2014. Member of the Board of Directors of the Chilean Society for Reproduction and Development: 2007-2008 (Treasurer), 2009-2012 (Director), 2012-2014 (Secretary)
2014-2015. Member of the Scientific Committee VI Congress Latin American Society of Maternal-Fetal Interaction SLIMP. Mar del Plata, April 2015.
2016-2017. Member of the Organizing Committee VII Congress Latin American Society of Maternal-Fetal Interaction SLIMP. Puerto Varas 2017.
2016-2018. Member of the Board of Directors of the Chilean Society of Reproduction and Development. Director for Santiago.
2019. Member of the Scientific Committee VIII Congress Latin American Society of Maternal-Fetal Interaction (SLIMP) - International Federation of Placenta Associations. Buenos Aires, March 2015.

ADMINISTRATION IN SCIENCE AND RESEARCH MANAGEMENT

2013. Member of the Ethics and Welfare Committee of Laboratory Animals of the School of Medicine, Pontificia Universidad Católica de Chile.
2014. Chair Ethics and Welfare Committee of Laboratory Animals of the School of Medicine, Pontificia Universidad Católica de Chile.
2014-2019. Member of the Ethical Scientific Committee on Care and Use of Animals and Research Safety, Pontificia Universidad Católica de Chile.
2018- 2019. Vice-president Ethical Scientific Committee on Care and Use of Animals and Research Safety, Pontificia Universidad Católica de Chile.
2019. Chair Ethical Scientific Committee on Care and Use of Animals and Research Safety, Pontificia Universidad Católica de Chile.
2014-present. Member National Accreditation Commission (CNA) for Postgraduate programs - Chile
2015-2019. Member of the Study Group in Biomedicine, National Fund for Scientific and Technological Development (FONDECYT), National Reseach Council.

PARTICIPATION IN SCIENTIFIC SOCIETIES

1998-present. Argentine Society of Biology
2007-present. Chilean Society of Reproduction and Development
2012-present. International Society for Developmental Origins of Health and Disease (DOHaD)
2015- present. Society for the Study of Reproduction.

PARTICIPATION AS REVIEWER

International journals
2009- present. Biological Research
2012- present. Reproduction Fertility and Development
2013-present. Reproduction.
2014- present. PLOS One, PLOS Genetics, Journal of Physiology and Biochemistry, Revista Médica de Chile, Biomedical Research, Biology of Reproduction.
2019- present. Diabetologia.

Funding Agencies
2011- present. National Council for Scientific and Technical Research (CONICET), Argentina.
2012- present. National Fund for Scientific and Technological Development (FONDECYT), National Reseach Council.
2015-present. Fund for the Promotion of Scientific and Technological Development (FONDEF)



 

Article (43)

Ovarian cholesterol efflux: ATP-binding cassette transporters and follicular fluid HDL regulate cholesterol content in mouse oocytes
Attenuation of atherogenic apo B-48-dependent hyperlipidemia and high density lipoprotein remodeling induced by vitamin C and E combination and their beneficial effect on lethal ischemic heart disease in mice
High density lipoprotein cholesterol and proteome in SR-B1 KO mice: lost in precipitation
INTAKE OF RED WINE GRAPE POMACE DECREASED ATHEROSCLEROSIS, ATTENUATED MYOCARDIAL DAMAGE AND INCREASED SURVIVAL IN A MURINE MODEL OF LETHAL CORONARY HEART DISEASE
Transcriptional profiling of embryos lacking the lipoprotein receptor SR-B1 reveals a regulatory circuit governing a neurodevelopmental or metabolic decision during neural tube closure
Deficient Vitamin E Uptake During Development Impairs Neural Tube Closure in Mice Lacking Lipoprotein Receptor SR-BI
Gugulipid causes hypercholesterolemia leading to endothelial dysfunction, increased atherosclerosis, and premature death by ischemic heart disease in male mice
Prolonged Activation of the Htr2b Serotonin Receptor Impairs Glucose Stimulated Insulin Secretion and Mitochondrial Function in MIN6 Cells
Efecto del ciprofibrato sobre el metabolismo del colesterol HDL y la capacidad antioxidante plasmática en el ratón
Serotonin- and Dopamine-Related Gene Expression in db/db Mice Islets and in MIN6 beta-Cells Treated with Palmitate and Oleate
ANTIOXIDANT DYSFUNCTIONALITY OF HIGH-DENSITY LIPOPROTEINS (HDL) IN DECOMPENSATED DIABETIC PATIENTS
Preventive Letter: Doubling the Return Rate After Gestational Diabetes Mellitus
Early Onset Intrauterine Growth Restriction in a Mouse Model of Gestational Hypercholesterolemia and Atherosclerosis
Excess cholesterol induces mouse egg activation and may cause female infertility
Human fertilization: Epididymal hCRISP1 mediates sperm-zona pellucida binding through its interaction with ZP3
Maternal Hypertriglyceridemia: A Link Between Maternal Overweight-Obesity and Macrosomia in Gestational Diabetes
Papel protector de las lipoproteínas de alta densidad en sepsis: aspectos básicos e implicancias clínicas
Perfil lipídico en mujeres embarazadas sanas de tres regiones de Chile
Physiological and pathological implications of cholesterol
Prenatal nicotine exposure enhances Cx43 and Panx1 unopposed channel activity in brain cells of adult offspring mice fed a high-fat/cholesterol diet
Protective role of high density lipoproteins in sepsis: basic issues and clinical implications
Spermatozoa from mice deficient in Niemann-Pick disease type C2 (NPC2) protein have defective cholesterol content and reduced in vitro fertilising ability
Developmental abnormalities in mouse embryos lacking the HDL receptor SR-BI
Fertilization Induces a Transient Exposure of Phosphatidylserine in Mouse Eggs
Evaluation of Testicular Sperm CRISP2 as a Potential Target for Contraception.
Apolipoprotein A-I deficiency does not affect biliary lipid secretion and gallstone formation in mice
Female infertility due to anovulation and defective steroidogenesis in NPC2 deficient mice
Life-giving caspases: revealing new roles during mouse embryo preimplantation development
Immunocontraceptive properties of recombinant protein DE: implications for the development of novel contraceptives
Participation of cysteine-rich secretory proteins (CRISP) in mammalian sperm-egg interaction
A novel function for CRISP1 in rodent fertilization: Involvement in sperm-zona pellucida interaction
Evidence for the involvement of testicular protein CRISP2 in mouse sperm-egg fusion
Participation of epididymal cysteine-rich secretory proteins in sperm-egg fusion and their potential use for male fertility regulation.
Sperm protein DE mediates gamete fusion through an evolutionarily conserved site of the CRISP family
Expression and structure-function analysis of de, a sperm cysteine-rich secretory protein that mediates gamete fusion.
Molecular mechanisms involved in mammalian fusion
Relationship between the association of rat epididymal protein “DE” with spermatozoa and the behavior and function of the protein
Bicarbonate is required for migration of sperm epididymal protein DE/CRISP-1 to the equatorial segment and expression of rat sperm fusion ability.
Evidence that human epididymal protein ARP plays a role in gamete fusion through complementary sites on the surface of the human egg
Human testicular protein TPX1/CRISP-2: localization in spermatozoa, fate after capacitation and relevance for gamete interaction
Molecular mechanisms involved in gamete interaction.
Morphologic and functional determinants of primordial and primary follicles in the monkey ovary.
Studies on the participation of epididymal sperm protein DE/CRISP-1 in egg activation.

EditorialMaterial (1)

Blood lipids during pregnancy: A progressively appreciated subject in basic and clinical research

Proyecto (10)

Magnetic resonance imaging technology for aging related diseases: brain, heart and vessels
Cholesterol is bad for eggs as much as eggs are bad for cholesterol.
Impact of high density lipoprotein metabolism in mouse oocyte and embryo physiology: underlying mechanisms, pathological implications and potential interventions
Role of Good Cholesterol HDL in Female Fertility
Unrelenting Macrosomia Despite Optimal Glycemic Control in Gestational Diabetes Mellitus: is an Excessive Rise of Maternal Serum Lipids the Link Between Prepregnancy Overweight and Excessive Fetal Growth?
Involvement of receptor-dependent high density lipoprotein metabolism on mouse female fertility and embryonic development
Contribution of the NPC2 cholesterol transport protein to steroid synthesis and follicular development in the ovary
Relevance of Niemann Pick C proteins in physiology and pathophysiology
Role of testicular protein TPX-1 in fertilization and its potential use to regulate fertility
Participation of a protein family of epididymal protein DE in the process of fertilization.

Review (4)

Advances in the physiological and pathological implications of cholesterol
La hipótesis de Pedersen no es suficiente: Otros nutrientes además de la glucosa explicarían la macrosomía fetal en pacientes diabéticas gestacionales con sobrepeso y buen control glicémico
Nutrients other than glucose might explain fetal overgrowth in gestational diabetic pregnancies
Mechanisms regulating hepatic SR-BI expression and their impact on HDL metabolism
60
Dolores Busso

Associate Professor

Centro de Investigación e Innovación Biomédica

Universidad de Los Andes

Santiago, Chile

21
Attilio Rigotti

Full Professor

Nutrition, Diabetes and Metabolism

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE - ESCUELA DE MEDICINA - DEPTO DE GASTROENTEROLOGÍA

Santiago, Chile

6
JOSE SANTOS

Full Professor

Department of Nutrition, Diabetes and Metabolism

PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

Santiago, Chile

6
Nicolás Santander

Assistant Professor

Instituto de Ciencias de la Salud

Universidad de O'Higgins

Rancagua, Chile

4
Attilio Rigotti

Full Professor

Nutrition, Diabetes and Metabolism

Pontificia Universidad Católica de Chile

Santiago, Chile

3
Andrea Leiva

Profesor Asociado

Facultad de Ciencias de la Salud

Universidad San Sebastián

Santiago, Chile

3
Silvana Zanlungo

Full Professor

Gastroenterology

Pontificia Universidad Católica de Chile

Santiago, Chile

3
Loni Berkowitz

Investigador postdoctoral

Nutrición, diabetes y metabolismo

Pontificia Universidad Católica de Chile

SANTIAGO, Chile

2
Susana Contreras

Assistant Professor

Nutrition and Dietetics

Universidad San Sebastián

Santiago, Chile

2
Pablo Irarrazaval

Professor

Electrical Engineering

Pontificia Universidad Católica de Chile

Santiago, Chile

2
ELISA BALBOA

Académico Investigador

Facultad de Medicina

Universidad Finis Terrae

SANTIAGO, Chile

2
María Mizgier

Postdoctorate

Universidad de Los Andes

Santiago, Chile

2
Victor Cortes

Associate professor

Nutrition, Diabetes and Metabolism

Pontificia Universidad Católica de Chile

Santiago, Chile

2
Sergio Uribe

Associated professor

Radiology

PONTIFICIA UNIVERSIDAD CATÓLICA

Santiago, Chile

2
Francisca Salas

Asistant Professor

Instituto de Ciencias de la Salud

Universidad de O'Higgins

Rancagua, Chile

2
Barbara Samith

Profesor Instructor Adjunto

Ciencias de la Salud

Pontificia Universidad Católica de Chile

Santiago, Chile

2
RICARDO MORENO

Profesor Titular

Fisiología

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE, FACULTAD DE CIENCIAS BIOLÓGICAS, DEPTO. DE FISIOLOGÍA

Santiago, Chile

2
Juan Orellana

Associate Professor

Departamento de Neurología

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

2
JOSE GALGANI

ASSOCIATE PROFESSOR

NUTRITION, DIABETES AND METABOLISM; AND UDA-CIENCIAS DE LA SALUD, NUTRICION Y DIETETICA

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

1
Pablo Mardones

Research Associate

PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

Santiago, Chile

1
Paola Llanos

Profesor Asistente

Instituto de Investigacion en Ciencias Biomédicas

FACULTAD DE ODONTOLOGIA. UNIVERSIDAD DE CHILE

Santiago, Chile

1
Alvaro Martínez

Director Asociado de Propiedad Intelectual y Transferencia Tecnológica

FUNDACIÓN CIENCIA PARA LA VIDA (FCV)

Santiago, Chile

1
Hugo Verdejo

Profesor Asociado

División de Enferemedades Cardiovasculares

Pontificia Universidad Católica de Chile

Santiago, Chile

1
valentina serrano

PROFESOR ASISTENTE

NUTRICION, DIABETES Y METABOLISMO

PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

SANTIAGO, Chile

1
Alberto Martin

Associate professor

Centro Ciencia & Vida

Universidad San Sebastián

Santiago, Chile

1
Rommy von Bernhardi

Full Professor

Neurology

DEPT. NEUROLOGY, FAC. MEDICINE PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

1
Jaime Eugenin

Full Professor

Biology

Universidad de Santiago de Chile

Santiago, Chile

1
Alejandro Godoy

Associate Professor

Centro de Biología Celular y Biomedicina

Universidad San Sebastián

Santiago, Chile

1
Claudio Vera

Assitant Professor

Obstetrics and Gynecology

PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE

Santiago, Chile

1
Fabián Jaña

Profesor Asistente

Departamento de Ciencias de la Salud

Universidad de Aysén

Coyhaique, Chile

1
Katherine Rivera

PhD (c) student in Medical Science

Pontificia Universidad Católica de Chile

Santiago, Chile

1
Tomas Perez-Acle

Full Professor

Computational Biology Lab

Fundación Ciencia para la Vida

Santiago, Chile

1
Guadalupe Echeverría

Manager

Center for Molecular Nutrition and Chronic Diseases

Pontificia Universidad Católica de Chile

Santiago, Chile

1
Eduardo Abbott

Profesor Asistente

Medicina Interna

Pontificia Universidad Católica de Chile

Santiago, Chile

1
Leandro Murgas

Bioinformático

Meristem Spa.

Huechuraba, Chile

1
Roberto Bravo

Assistant Professor

Institute of Nutrition and Food Technology (INTA), Universidad de Chile

Santiago, Chile