Man

Ruben Dario Almada

PRINCIPAL INVESTIGATOR

CENTRO DE ESTUDIOS AVANZADOS EN FRUTICULTURA

Rengo, Chile

Líneas de Investigación


FUNCTIONAL GENOMICS, PLANT DEVELOPMENT, ENVIRONMENTAL STRESSES, FRUIT TREES.

Educación

  •  Plant Genetic Engineering, UNIVERSIDAD DE TALCA. Chile, 2009
  •  GENETICIST, UNIVERSIDAD NACIONAL DE MISIONES. Argentina, 2002

Experiencia Académica

  •   VISITING PROFESSOR Other

    UNIVERSIDAD DE O´HIGGINS

    CIENCIAS AGRONOMICAS Y VETERINARIAS

    RANCAGUA, Chile

    2019 - 2019

  •   ASSOCIATED PROFESSOR PLANT DEVELOPMENT Part Time

    UNIVERSIDAD DE TALCA

    INSTITUTE OF BIOLOGY

    TALCA, Chile

    2019 - 2019

  •   ASSOCIATED PROFESSOR PLANT DEVELOPMENT Part Time

    UNIVERSIDAD DE TALCA

    INSTITUTE OF BIOLOGY

    TALCA, Chile

    2020 - 2020

Experiencia Profesional

  •   PRINCIPAL INVESTIGATOR Full Time

    CENTRO DE ESTUDIOS AVANZADOS EN FRUTICULTURA

    RENGO, Chile

    2010 - A la fecha

Formación de Capital Humano


7 undergraduate thesis were mentored in topics related to plant development and plant molecular biology in stone fruit trees species (Prunus genus, Rosaceae family).
1 PhD thesis co-advised in the PhD in Botany from the Universidad de Concepción (Chile) about the environmental factors that regulate the mycorrhyza communities in the Chilean sclerophyll forest.
1 PhD thesis co-advised in the PhD in Program of Post-Graduation in Plant Physiology from the Universidade Federal de Pelotas (Brazil)
1 Postdoc supervised (FONDECYT 3160316) related to the use of Genotyping by Sequencing (GBS) in plant breeding selection of stone fruit rootstocks.
Participation in several Master and PhD thesis evaluation committees (Universidad de Talca, Chile; Universidad Federal de Pelotas, Brasil).
Participation as Scientific Project evaluator in FONDECYT/ANID/CHILE and FONCYT/CONICET/ARGENTINA.


Difusión y Transferencia


Research stays:
-Plant Physiology Lab, Universidad Nacional de Río Cuarto (UNRC, Argentina) PI: Dra. Virginia Luna. Period: 1 month, year: 2018. Activity: Determination of Hormone levels in Sweet Cherry floral buds by HPLC/MS-MS.
-Plant Physiology Lab, Universidad Federal do Pelotas (UFPel, Brasil) PI: Dr. Valmor Bianchi. Period: 1 week, year: 2019. Activity: Research collaboration in plant molecular biology in Prunus rootstocks. Seminar talk for Master and PhD students about the molecular responses of Prunus rootstocks to root hypoxia. Also, a research collaboration agreement was signed between CEAF-UFPel .
-Plant Molecular Lab, USDA/ARS, Corvallis, Oregon (USA) PI: Ruth Martin. Period: 3 months, year: 2014. Activity: Functional characterization of transcription factor genes isolated from Prunus roostocks.

Seminar talks and organization:
-Invited as main speaker in the XVIII Brazilian Congress of Plant Physiology and I Ibero-Latinamerican Congress of Plant Biology to be held in Porto Alegre, Brazil, (2021, September 19-22).
-Seminar talk: Molecular and hormonal basis of floral bud development in Sweet cherry (Prunus avium L.). Institute of Biology, Universidad de Talca, Chile. 2018.
-Seminar organization: Stone fruit rootstocks under a global climate change scenario: an Iberoamerican perspective. Rancagua, Chile. 11/14/2017. The speakers came from Spain, Brasil and Chile: TO the seminar assited undergradute and PhD student, growers, nurseries and consultants.
-Seminar talk: Transcriptome sequencing of Prunus sp. rootstocks roots to identify candidate genes involved in the response to root asphyxia. PhD Program in Plant Physiology, Universidad Federal de Pelotas, Brasil. 10/25/2017.
-Seminar talk: "Research and Science", “Flowering induction in sweet cherry (Prunus avium L.): a deep insight into the transcriptome and hormonal regulation of floral bud development”.Red de Gestión Tecnológica para el Agro, RGTA y PAR Explora O’Higgins. 12/16/2016.

Conference posters:
23 posters in Chilean Conferences related to Plant Biology
11 posters in International Conferences related to Plant Biology

Congress Organization
VIII Plant Biology Congress, Organization Committe-Chilean Society of Plant Biology. Pucón, Chile. 12/2-5/2013.

Workshop Organization
I International Workshop "Plant Rootstock Breeding with emphasis in stone fruit trees". The speakers came from Spain (Dra. Marian Moreno, CSIC; Dr. Jorge Pinochet, Agromillora), Brasil (Dr. Valmor Bianchi, UFPEL) and Chile (Dr. Nicola Fiore, UChile; Dra. Verónica Guajardo, CEAF): To the workshop assisted PhD students, Researchers, growers, nurseries and plant breeders. 11/14/2017-11/16/2017.

Science Outrech in the Scholar Society
-Speaker and co-organizer of Workshop: "Plant Cytogenetics and chromosomes". To the workshop assited teachers from public schools and they learned about the use of plant chromosomes and cytogenetics as tools to teach basic aspect of DNA organization and mitosis. In the International Seminar Scientific Education: a LatinAmerican perspective. PAR-EXPLORA O´higgins-CONICYT. Rancagua, 08/30/2016.


Premios y Distinciones

  •   Best Poster Award

    SOCIEDAD DE GENETICA DE CHILE

    Chile, 2021

    Best poster Award: Beltrán et al. (2021) GENES DEL SISTEMA T3SS EN LA POBLACIÓN DE Pseudomonas syringae pv. syringae ASOCIADO AL CEREZO (Prunus avium L.) EN CHILE./T3SS system genes in Pseudomonas syringae pv. syringae populations associated with cherry orchards in Chile.


 

Article (20)

Prunus Hexokinase 3 genes alter primary C-metabolism and promote abiotic stress tolerance in Arabidopsis transgenic plants
Comparative transcriptomic analysis reveals novel roles of transcription factors and hormones during the flowering induction and floral bud differentiation in sweet cherry trees (Prunus avium L. cv. Bing)
Genome-wide SNP identification in Prunus rootstocks germplasm collections using Genotyping-by-Sequencing: phylogenetic analysis, distribution of SNPs and prediction of their effect on gene function
Identifying and validating housekeeping hybrid Prunus spp. genes for root gene-expression studies
Genome-wide identification of single nucleotide polymorphisms (SNPs) and molecular characterization of Prunus rootstock germplasm using a genotyping-by-sequencing (GBS) approach
Aging gene pathway of microRNAs 156/157 and 172 is altered in juvenile and adult plants from in vitro propagated Prunus sp
Exogenous GABA application transiently improves the tolerance to root hypoxia on a sensitive genotype of Prunus rootstock
Identification and characterization of microsatellite loci in maqui (Aristotelia chilensis [Molina] Stunz) using next-generation sequencing (NGS)
Transcriptome sequencing of Prunus sp rootstocks roots to identify candidate genes involved in the response to root hypoxia
Physiological and morphological responses of Prunus species with different degree of tolerance to long-term root hypoxia
Class 1 non-symbiotic and class 3 truncated hemoglobin-like genes are differentially expressed in stone fruit rootstocks (Prunus L.) with different degrees of tolerance to root hypoxia
Comparative cytogenetics in Cyclopogon (Orchidaceae)
Occurrence of aphidborne viruses in southernmost South American populations of Fragaria chiloensis ssp chiloensis
Characterization of Genetic Diversity of Stone Fruit Rootstocks Used in Chile by Means of Microsatellite Markers
Epigenetic repressor-like genes are differentially regulated during grapevine (Vitis vinifera L.) development
Epigenetic repressor-like genes are differentially regulated during grapevine (Vitis vinifera L.) development
Chromosome studies in Orchidaceae from Argentina
VvCO and VvCOL1, two CONSTANS homologous genes, are regulated during flower induction and dormancy in grapevine buds
Chromosome studies in Orchidaceae from Argentina, II
Chromosome studies in Orchidaceae from Argentina, III

Proyecto (16)

“Desarrollo y validación de un servicio innovador para la identificación de especies frutales de importancia económica a través de la implementación de una técnica biotecnológica basada en qPCR-HRM”,
“Development and validation of a novel sunscreen with active components to reduce the incidence of double fruits in cherry
Fortalecimiento de las capacidades científicas y tecnológicas del Programa de Mejoramiento Genético y Líneas de investigación de CEAF para incrementar la vinculación científica, la prestación de servicios y la visibilidad del Centro Regional
• EXTREMOPHYTES: THE NEW PROMISING MODELS FOR DISCOVERING STRESS TOLERANCE MECHANISMS AND BIOACTIVE COMPOUNDS
Dissecting the genetic architecture of color inheritance in sweet cherry (P. avium L.) fruit (skin and flesh) by the means of QTLs, eQTLs and expression of key-candidate genes, during ripening
Flowering induction in sweet cherry (Prunus avium l.)=> a deep insight into the transcriptome and hormonal regulation of floral bud development
Breeding and genetic analysis in Prunus rootstocks and peach varieties
Caracterización de la diversidad genética de portainjertos de frutales de carozo (Prunus L.) utilizando SNPs identificados a través de Genotyping-by-sequencing y genotipado de alelos S
Centro de Estudios Avanzados en Fruticultura (CEAF), Región de O´Higgins, Etapa Continuidad
Waterlogging in Prunus rootstocks: a deep physiological insight of the hormonal regulation on root water uptake through aquaporins
WATERLOGGING IN PRUNUS ROOTSTOCKS=> A DEEP PHYSIOLOGICAL INSIGHT OF THE HORMONAL REGULATION ON ROOT WATER UPTAKE THROUGH AQUAPORINS
Desarrollo e implementación de un banco de polen crio-preservado de Prunus spp. para Chile- VI Concurso de Fortalecimiento de Centros Regionales de Desarrollo Científico y Tecnológico
Fortalecimiento de las capacidades de conservación y estudio de la biología de polen. Centro de Estudios Avanzados de Fruticultura
CHARACTERIZATION OF ALLELIC DIVERSITY OF CANDIDATE GENES ASSOCIATED TO HYPOXIA RESPONSE AND IDENTIFICATION OF ALLELES ASSOCIATED WITH TOLERANCE USING QTL ANALYSIS IN SEGREGATING PROGENY, FOR PRUNUS SPP. ROOTSTOCK DEVELOPMENT
SIGNAL TRANSDUCTION IN RESPONSE TO ROOT HYPOXIA=> CHARACTERIZATION OF ETHYLENE-RESPONSIVE TRANSCRIPTIONAL FACTORS (ERF) GENES IN PRUNUS ROOTSTOCKS
Implementación de una plataforma de microscopía para estudios histológicos y de genómica (hibridación in situ) en frutales
20
Ruben Almada

PRINCIPAL INVESTIGATOR

GENOMICS

CENTRO DE ESTUDIOS AVANZADOS EN FRUTICULTURA

Rengo, Chile

6
Boris Sagredo

INIA-RAYENTUÉ

Rengo, Chile

5
Paula Pimentel

Coordinator of Plant Stress Physiology laboratory

Centro de Estudios Avanzados en Fruticultura

Rengo, Chile

4
Manuel Pinto

ACADÉMICO

INSTITUTO DE CIENCIAS AGRONOMICAS Y VETERINARIAS

Universidad de O'Higgins

San Fernando, Chile

4
Patricio Hinrichsen

Investigador

INIA CENTRO REGIONAL LA PLATINA

Santiago, Chile

4
Pamela Rojas

Doctora en Ciencias, Coordinadora Científica

Vicerrectoría de Investigación y Doctorado

Universidad Autónoma de Chile

Santiago, Chile

3
Ariel Salvatierra

Investigador

Genómica Vegetal

Centro de Estudios Avanzados en Fruticultura

Rengo, Chile

2
Adriana Bastias

DOCENTE

FAC. CS. SALUD

UNIVERSIDAD AUTONOMA DE CHILE

Santiago, Chile

1
MARIA TERESA PINO

Investigadora y Jefa de Cooperación Internacional para I+D+i

Dirección Nacional

INSTITUTO DE INVESTIGACIONES AGROPECUARIAS INIA

Santiago, Chile

1
Alejandro Maass

Main Researcher

Center for Mathematical Modeling

Universidad de Chile

Santiago, Chile

1
Verónica Guajardo

Principal Researcher, Genetic Breeding Research Group

Genetic Breeding

Centro de Estudios Avanzados en Fruticultura (CEAF)

Rengo, Chile

1
Peter Caligari

Science Advisor

CGNA

Temuco, Chile

1
Alex Di Genova

Associate professor

Engineering sciences

Universidad de O'Higgins

Rancagua, Chile

1
Dante Travisany

Profesor Asociado

Facultad de Ingeniería y Negocios

Universidad de Las Américas

Santiago, Chile