Biología molecular y celular vegetal. Tráfico de endomembranas. Interacción Planta-Patógeno. Sanidad Vegetal. Diagnóstico Molecular de fitopatógenos.

(1) Coguía estudiantes de pregrado (2022-2024)
Macarena Carreño, memoria de título para optar al título de Ingeniero en Ciencias Agronómicas
Boris Ponce, memoria de título para optar al título de Ingeniero en Ciencias Agronómicas
Nicole Torres, memoria de título para optar al título de Ingeniero en Ciencias Agronómicas
Gabriel Bastías, memoria de título para optar al título de Ingeniero en Ciencias Agronómicas

(2) Cotutor unidades de investigación estudiantes de pregrado
Pablo Santiago, estudiante de Ingeniería en Biotecnología Molecular, Facultad de Ciencias, Universidad de Chile (2022)

(3) Tutorías estudiantes de pregrado
Nicolás Cabrera, estudiante de Bioquímica, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile (2023)
Paula Sierra, estudiante de Bioquímica, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile (2023)
Claudio Muñoz, estudiante de Ingeniería en Biotecnología Molecular, Facultad de Ciencias, Universidad de Chile (2023)

Partición en proyectos como asistente de investigación o investigador:
- Proyecto FIA PYT-2023-0577 (2023 a la fecha)
- Proyecto FIA PYT-2023-0583 (2024 a la fecha)
- Proyecto FIA PYT-2020-0208 (2020-2022)
- Proyecto FONDECYT 1170950 (2019)
- Proyecto FIC 40008890-0 (2019-2021)
- Proyecto FIA PYT-2016-0243 (2016-2018)
- Proyecto VIU 110058 (2012)

Membresía en sociedades científicas
Sociedad Chilena de Fitopatología (SOCHIFIT), desde 2024.

Participación en patentes de invención
Sistema de diagnóstico: Check Fast Botrytis (2023), patente en trámite

Extensión y difusión
- Artículo de extensión en el medio agrícola RedAgrícola (2020)
- Participación Proyecto Valentín Letelier “MicroMundo” (2021) Directo: Dr. Jorge Toledo, Facultad de Medicina
- Participación Feria Científica Colegio Oxford (educación básica), Maipú (2023)
- Expositor invitado encuentro “Avances recientes: cerezo” organizado por UC Davis Chile Life Sciences Innovation Center (2023)

Participación en congresos internacionales
Osorio-Navarro, C., Norambuena, L. Inhibition of Clathrin-Mediated Endocytosis through a transmissible signal precedes colonization by Phytophthora cinnamomi in Arabidopsis thaliana root. The 24th International Conference on Plant Growth Substances, KOREA, Hico, Gyeongju, 2023.

Osorio-Navarro, C., Norambuena, L. Arabidopsis thaliana Clathrin-Mediated Endocytosis is Critical for the Successful Infection the Pathogen Oomytcete Phytophthora cinnamomi. 7th Pan American Plant Membrane Biology Workshop, MEXICO, Telchac-Mérida, 2023.

Osorio-Navarro, C., Copier, C., Durán, F., Azocar, M., Gutiérrez, G., Donoso, E., Silva, H., Auger, J., Esterio, M. Real-time design of Botrytis cinerea control programs based on direct qPCR-HRM (High-Resolution Melting) fungicide-resistance genotyping from infected plant tissue. 10th International Table Grape Symposium. SOUTH AFRICA, Cape Town, 2023.

Gutiérrez, G., Osorio-Navarro, C., Copier, C., Durán, F., Azocar, M., Kauer, P., Donoso, E., Merino, M., Rivara, P., Sepúlveda, J.C., Silva, H., Auger, J., Esterio, M. Fungal species screening from Vitis vinifera native microbiome determines the Botrytis control ability of Trichoderma gambsii strains. 1st joint BOTRYTIS-SCLEROTINIA Symposium, FRANCE, Avignon, 2022.

Esterio, M., Rubilar, M., Copier, C., Osorio-Navarro, C., Valdes, S., Rivara, P., Scalliet, G., Auger, J. Adepidyn™ baseline sensitivity against Botrytis cinerea isolates recovered from table grapes and wine grapevines of the main Chilean producing regions. 1st joint BOTRYTIS-SCLEROTINIA Symposium, FRANCE, Avignon, 2022.

Copier, C., Silva, H., Osorio-Navarro, C., Maldonado, J., Auger, J., Esterio, M. Changes along the Mrr1 gene correlate with reduced fludioxonil sensitivity in Botrytis cinerea. 1st joint BOTRYTIS-SCLEROTINIA Symposium, FRANCE, Avignon, 2022.

Osorio-Navarro, C., Copier, C., Durán, F., Azocar, M., Gutiérrez, G., Kauer P., Donoso, E., Merino, M., Rivara, P., Sepúlveda, J.C., Silva, H., Auger, J., Esterio, M. Real-time design of Botrytis cinerea control programs based on plant tissue detection of fenhexamid- and boscalid-resistance genotypes using qPCR-HRM (High Resolution Melting). 1st joint BOTRYTIS-SCLEROTINIA Symposium, FRANCE, Avignon, 2022.

Osorio-Navarro, C., Carreras, C., Esterio, M., Auger, J. Botrytis species detection by High Resolution Melting (HRM) analysis reveals the presence of Botrytis byssoidea as a new rotting agent in kiwifruit. BotrySclero Webinar, FRANCE, Avignon, 2021.

Auger, J., Osorio-Navarro, C., Pozo, L., Rubilar, M., Esterio, M. Outbreak of young grapevines diseases in new table grape varieties in Chile. XI International Workshop on Grapevine Trunk Disease, CANADA, British Columbia, 2019.

Osorio-Navarro, C., & Norambuena, L. Endocytic cellular trafficking, an unexpected contributor of the vacuolar configuration process in Arabidopsis thaliana. 6th PanAmerican Plant Membrane Biology Workshop, CANADA, Vancouver, 2018.

Esterio, M., Osorio-Navarro, C., Copier, C., Rubilar, M, Pizarro, L., Auger, J. Identification of Erg27 and SdhB–mutations by Melting Curve Analysis as a strategy for detection of fenhexamid and boscalid sensitivity levels of Botrytis cinerea isolates on Chilean table grape. LVII Meeting of APS Caribbean Division-XIX Congreso Latinoamericano de Fitopatología-XXV Congreso de la Sociedad Chilena de Fitopatología, CHILE, Chillán, 2017.

Hermosilla, A., Auger, J., Copier, C., Osorio-Navarro, C., Rubilar, M., Esterio, M. Genetic and phenotypic characterization of Botrytis cinerea chilean isolates with different tebuconazole sensitivity levels. LVII Meeting of APS Caribbean Division-XIX Congreso Latinoamericano de Fitopatología-XXV Congreso de la Sociedad Chilena de Fitopatología, CHILE, Chillán, 2017.

Osorio-Navarro, C., & Norambuena, L. The vacuole configuration requires functional clathrin-mediated endocytosis in Arabidopsis thaliana roots cells. 19th European Network for Plant Endomembrane Research Meeting, FRANCIA, Bordeaux, 2016.

Pizarro, L., Osorio-Navarro, C., Copier, Ch., Araneda, M.J.,
Harmsen, I., Hermosilla, A., Rubilar, M., Auger, J.
Esterio, M. A single probe qPCR system to identify Botrytis cinerea hydroxyanilides- and carboxamides-resistant isolates. XVII International Botrytis Symposium, CHILE, Santa Cruz, 2016.

Osorio-Navarro, C. & Norambuena, L. Clathrin-dependent plasma Membrane internalization impacts the structure of the vacuole in Arabidopsis thaliana. 5th PanAmerican Plant Membrane Biology Workshop, CHILE, San Pedro de Atacama, 2015.

Pizarro L., Osorio-Navarro C., Vergara, A., Bidegain, A., Urbina, D., Rojas-Pierce, M., Gutiérrez R.A., Hicks ,G., Norambuena, L. System biology approach reveals transcriptional factors involved in endomembrane trafficking regulation in Arabidopsis thaliana. 5th PanAmerican Plant Membrane Biology Workshop, CHILE, San Pedro de Atacama, 2015.

Pizarro, L., González, C., Osorio-Navarro, C., Vergara, A., Hicks, G., Gutiérrez, R.A., Norambuena, L. Trafficking Regulatory Network reveals transcriptional endomembrane trafficking regulation. European Network for Plant Endomembrane Research Meeting, BELGICA, Ghent, 2013.

Participación en congresos nacionales
Carreño, M., Osorio-Navarro, C., Durán, F., Azócar, M., Estrada, V., Auger, J., Esterio, M. Changes in the Erg27 promoter region are associated with differential sensitivity to fenhexamid and fenpyrazamine in Botrytis cinerea. XXX Congreso de la Sociedad de Fitopatología de Chile. CHILE, Santa Cruz, 2023.

Ponce, B., Osorio-Navarro, C., Durán, F., Azocar, M., Auger, J., Esterio, M. Mutations in SdhC and SdhD of Botrytis cinerea, revealed by qPCR-HRM, are associated with differential sensitivity to boscalid, fluopyram and isofetamide. XXX Congreso de la Sociedad de Fitopatología de Chile. CHILE, Santa Cruz, 2023.

Osorio-Navarro, C., Fernández, Y., Henríquez, J.L., Norambuena, L. Endocytic trafficking is reprogramed in Arabidopsis root during oomycete biotrophic interaction. XLV Annual Meeting of the Society for Biochemistry and Molecular Biology of Chile. CHILE, Cuncumén, 2022.

Galleguillos, C., Osorio-Navarro, C., Cifuentes, D., Norambuena, L. Transcriptional networks of bZIP25 regulates endocytic trafficking in Arabidopsis thaliana. XLV Annual Meeting of the Society for Biochemistry and Molecular Biology of Chile. CHILE, Cuncumén, 2022.

Osorio-Navarro, C., Pozo, L., Briceño, N., Rubilar, M., Azocar, M., Esterio, M., Auger, J. Transcriptional induction of defense response genes in fruit trees under chitosan and salicylic acid (Actigen®) treatment. XXIX Congreso de la Sociedad de Fitopatología de Chile. CHILE, Valparaíso, 2022.

Auger, J., Durán, F., Briceño, N., Osorio-Navarro, C., Esterio, M. Arambarria destruens, Rajchenb & Pildain, associated with soft wood rot in Vitis vinifera, Actinidia deliciosa and Prunus domestica in Chile. XXIX Congreso de la Sociedad de Fitopatología de Chile. CHILE, Valparaíso, 2022.

Sáez, C., Briceño, N., Azocar, M., Osorio-Navarro, C., Estrada, V., Durán, F., Esterio, M., Auger, J. Susceptibility of the main sweet cherry cultivars (Prunus avium L.) to Cytospora leucostoma and Calosphaeria pulchella in Chile. XXIX Congreso de la Sociedad de Fitopatología de Chile. CHILE, Valparaíso, 2022.

Copier, C., Silva, H., Osorio-Navarro, C., Maldonado, J., Auger, J., Esterio, M. Changes along the Mrr1 gene correlate with reduced fludioxonil sensitivity in Botrytis cinerea. XXIX Congreso de la Sociedad de Fitopatología de Chile. CHILE, Valparaíso, 2022.

Esterio, M., Osorio-Navarro, C., Copier, C., Durán, F., Azocar, M., Gutiérrez, G., Briceño, N., Kauer, P., Donoso,
E., Merino, M., Rivara, P., Sepúlveda, J.C., Silva, H., Auger, J. “Check Fast Botrytis, know now to apply tomorrow” An accurate and timely diagnostic tool to combat Botrytis cinerea in real time. XXIX Congreso de la Sociedad de Fitopatología de Chile. CHILE, Valparaíso, 2022.

Esterio, M., Gutiérrez, G., Azocar, M., Osorio-Navarro, C., Estrada, V., Briceño, N., Kauer, P., Tapia, M., Auger, J. Mefentrifluconazole (Cevya) Baseline sensitivity of Botrytis cinerea isolates recovered from table grape from Chilean Central Valley. XXIX Congreso de la Sociedad de Fitopatología de Chile. CHILE, Valparaíso, 2022.

Esterio, M., Rubilar, M., Copier, C., Azócar, M., Osorio-Navarro, C., Valdes, S., Scalliet, G., Auger, J. Adepidyn™ baseline sensitivity against Botrytis cinerea isolates recovered from table grapes and wine grapes located in Chilean producing regions. XXIX Congreso de la Sociedad de Fitopatología de Chile. CHILE, Valparaíso, 2022.

Osorio-Navarro, C., Sotomayor, V., Toledo, J., Norambuena, L. Sucrose promotes Clathrin-Mediated Endocytosis in Arabidopsis thaliana. XLIV Annual Meeting of the Society for Biochemistry and Molecular Biology of Chile. online, 2021.

Gutiérrez, G., Osorio-Navarro, C., Copier, C., Azocar, M., Durán, F., Auger, J., Esterio, M. Fungal community of table grape flowers and berries (Vitis vinifera cv Thompson Seedless) as potential biological control agents. XXVIII Congreso de la Sociedad de Fitopatología de Chile. CHILE, Concepción, 2021.

Osorio-Navarro, C., Carreras, C., Esterio, M., Auger, J. Botrytis spp. detection system using qPCR-HRM (High Resolution Melting) reveals the presence of a new cryptic species in kiwifruit. XXVIII Congreso de la Sociedad de Fitopatología de Chile. CHILE, Concepción, 2021.

Osorio-Navarro, C., Durán, F., Copier, C., Azocar, M., Gutiérrez, G., Auger, J., Esterio, M. In planta detection of Botrytis cinerea fenhexamid- and boscalid-resistance genotypes by qPCR-HRM (High Resolution Melting). XXVIII Congreso de la Sociedad de Fitopatología de Chile. CHILE, Concepción, 2021.

Bustamante, M., Osorio-Navarro, C., Henríquez, J.L. Diversity of Colletotrichum species associated with anthracnose of the avocado fruits in Chile. I Encuentro Chileno de Micología. CHILE, Santiago, 2019.

Cifuentes Aguilar, D., Pavez Malmborg F., Osorio-Navarro, C., Pizarro, L., Norambuena, L. bZIP25 as a negative regulator in endocytic trafficking and a modulator in lateral root development in response to nutritional deficit. XLII Annual Meeting of the Society for Biochemistry and Molecular Biology of Chile. CHILE, Iquique, 2019.

Carreras, C., Osorio-Navarro, C., Esterio, M., Auger, J. Study of populations of Botrytis spp. during the Actinidia deliciosa cv. Hayward fruit development in VI Region of Chile. XXVI Congreso de la Sociedad de Fitopatología de Chile. CHILE, Valdivia, 2018.

Esterio, M., Copier, C., Rubilar, M., Osorio-Navarro, C., Azócar, M., Auger J. Increase in the number of applications of fludioxonil per season and the risk of loss of sensitivity of Botrytis cinerea: a threat to the industry of table grape for export in Chile. XXVI Congreso de la Sociedad de Fitopatología de Chile. CHILE, Valdivia, 2018.

Copier C., Auger, J., Osorio-Navarro, C., Rubilar, M., Pizarro, L., Esterio, M. Population dynamics of Botrytis cinerea Chilean isolates with different Hydroxyanilides and Carboxamides sensitivity levels. XXVI Congreso de la Sociedad de Fitopatología de Chile. CHILE, Valdivia, 2018.

Rubilar, M., Auger, J., Copier, C., Osorio-Navarro, C., Azócar, M., Pizarro, L., Esterio, M. Optimun Botrytis cinerea control programs based on Hydroxyanilides and Carboxamides sensitivity levels. XXVI Congreso de la Sociedad de Fitopatología de Chile. CHILE, Valdivia, 2018.

Azócar M., Auger J., Copier C., Osorio-Navarro C., Rubilar M., Pizarro L., Esterio, M. Phenotypical characterization of Botrytis cinerea Chilean isolates with erg27 and sdhB genes mutations. XXVI Congreso de la Sociedad de Fitopatología de Chile. CHILE, Valdivia, 2018.

Osorio-Navarro, C., Norambuena, L. Clathrin-mediated trafficking is required for central vacuole configuration in Arabidopsis thaliana. XI Reunión de Biología Vegetal. Chilean Society of Plant Biologists. CHILE, Chillán, 2016.

Esterio, M., Osorio-Navarro, C., Rubilar, M., Copier, Ch., Hermosilla, A., Harmsen, I., Pizarro, L., Auger, J. Design of optimal Botrytis cinerea control programs by a unique probe fluorescent system for mutation recognition in Erg27 and SdhB. 67° Congreso de la Sociedad Agronómica de Chile y 14° de la SOCHIFRUT. CHILE, Santiago, 2016.

Osorio, C., Pizarro, L., Norambuena, L. Endocytic trafficking impacts vacuole structure affecting root architecture in Arabidopsis thaliana. XXXVII Annual Meeting of the Society for Biochemistry and Molecular Biology of Chile. CHILE, Puerto Varas, 2014.

Osorio, C., Pizarro, L., Norambuena. L. bZIP25 transcription factor participates in lateral root development regulating clathrin-mediated endocytosis. VIII Plant Biology Meeting, CHILE, Pucón, 2013.

Pizarro, L., Osorio, C., Vergara, A., Rojas-Pierce, M., Gutiérrez, R., Hicks, G., Norambuena, L. System biology in Arabidopsis thaliana identifies a novel transcription factor involved in protein trafficking and regulation of endocytosis. VIII Plant Biology Meeting, CHILE, Pucón, 2013.

Osorio, C., Morales, S., Pérez-Henríquez, P., Pizarro, L., Norambuena, L. New evidence of Lateral Root Development by Accelerated Endocytosis: Genetic and Pharmacological Approach. VII Plant Biology Meeting, CHILE, Pucón, 2012.

Pizarro, L., Osorio, C., Norambuena, L. Endocytosis regulation at transcriptional level in Arabidopsis thaliana: bZIP25 a novel functional transcription factor of endocytosis (2012). Annual Meeting of the Society for Biochemistry and Molecular Biology of Chile. CHILE, Puerto Varas, 2012.