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Daniel Martín Veraguas Dávila

Assistant Professor

Universidad Católica del Maule

Curicó, Chile

Líneas de Investigación


Embryology, Animal Reproduction, In Vitro Embryo Production, Animal Cell Culture, Animal Cloning. Mainly focused on the in vitro production of feline embryos as a conservation method for endangered species.

Educación

  •  Doctor en Ciencias Veterinarias - Reproducción Animal, UNIVERSIDAD DE CONCEPCION. Chile, 2018
  •  Médico Veterinario, UNIVERSIDAD DE CONCEPCION. Chile, 2015
  •  Licenciado en Ciencias Veterinarias, UNIVERSIDAD DE CONCEPCION. Chile, 2012

Experiencia Académica

  •   Associated Professor - Academic Researcher Full Time

    UNIVERSIDAD CATOLICA DEL MAULE

    Ciencias Agrarias y Forestales

    Curicó, Chile

    2023 - A la fecha

  •   Professor Part Time

    UNIVERSIDAD ANDRES BELLO

    Curicó, Chile

    2021 - A la fecha

Experiencia Profesional

  •   Postdoctoral Researcher Full Time

    UNIVERSIDAD DE CONCEPCIÓN

    Chillán, Chile

    2020 - 2023

  •   Researcher Full Time

    UNIVERSIDAD DE CONCEPCIÓN

    Chillán, Chile

    2017 - 2020

Formación de Capital Humano


Direction of Degree Thesis, Title Memories

Producción in vitro de embriones en la conservación de felinos silvestres y la situación actual en Chile. Tesista. Camila Andaliesy Zapata Rojas. Medicina Veterinaria. Universidad Andrés Bello. 20223.


Premios y Distinciones

  •   Beca CONICYT Doctorado Nacional 2014

    CONICYT

    Chile, 2014

    Beca CONICYT para doctorado en Chile

  •   CANDES Trainee Travel Award

    International Embryo Technology Society (IETS) - CANDES

    Estados Unidos, 2020

    Trainee award from the Companion Animals Non Domestic and Endangered Species (CANDES)


 

Article (16)

Zona pellucida removal modifies the expression and release of specific microRNAs in domestic cat blastocysts
Analysis of trophectoderm markers in domestic cat blastocysts cultured without zona pellucida
Short Communication: Pre-implantation bovine embryos secrete small extracellular vesicles that contain embryonic DNA useful for genetic analysis
Domestic cat embryos generated without zona pellucida are capable of developing in vitro but exhibit abnormal gene expression and a decreased implantation rate
Nanoparticles from culture media are internalized by in vitro-produced bovine embryos and its depletion affect expression of pluripotency genes
Embryo aggregation allows the production of kodkod (Leopardus guigna) blastocysts after interspecific SCNT
Evaluation of extracellular vesicles and gDNA from culture medium as a possible indicator of developmental competence in human embryos
In vitro and in vivo development of domestic cat embryos generated by in vitro fertilization after eCG priming and oocyte in vitro maturation
Differentiation and multipotential characteristics of mesenchymal stem cells derived from adipose tissue of an endangered wild cat (Leopardus guigna)
The expression level of SOX2 at the blastocyst stage regulates the developmental capacity of bovine embryos up to day-13 of in vitro culture
eCG stimulation in domestic cats increases the expression of gonadotrophin-induced genes improving oocyte competence during the non-breeding season
Cell cycle synchronization and analysis of apoptosis-related gene in skin fibroblasts from domestic cat (Felis silvestris catus) and kodkod (Leopardus guigna)
FSH stimulation of anestrous cats improves oocyte quality and development of parthenogenetic embryos
Splitting of IVP bovine blastocyst affects morphology and gene expression of resulting demi-embryos during in vitro culture and in vivo elongation
Cheetah interspecific SCNT followed by embryo aggregation improves in vitro development but not pluripotent gene expression
The Endometrium of Cycling Cows Contains Populations of Putative Mesenchymal Progenitor Cells

ConferencePaper (1)

Evaluación del Desarrollo In Vitro e In Vivo de Embriones de Gato Doméstico Generados por Fecundación In Vitro Utilizando Ovocitos de Gatas Tratadas con eCG

ConferencePoster (20)

33 Domestic cat embryos cultured without the zona pellucida have an altered protein pattern at the blastocyst stage
29 The in vitro culture of domestic cat embryos without zona pellucida increases the expression of YAP1 and EOMES at the blastocyst stage
Altered expression of specific miRNAs in domestic cat blastocysts cultured without the zona pellucida
2 Noninvasive method for bovine embryo sexing through the analysis of DNA content in extracellular vesicles
25 Effect of roscovitine on the cumulus cells expansion, oocyte maturation and in vitro development of domestic cat embryos generated by in vitro fertilization
T166 Gene expression analysis of trophectoderm markers in domestic cat blastocysts cultured with and without zona pellucida
Effect of roscovitine on the developmental competence of domestic cat embryos produced by parthenogenetic activation, in vitro fertilization and somatic cell nuclear transfer
22 The zona pellucida is required for normal development of in vitro-produced cat embryos
80 Evaluation of extracellular vesicles from culture medium of human embryos as a possible method of pre-implantation genetic diagnosis
Effect of the zona pellucida removal on the developmental competence of domestic cat embryos generated by in vitro fertilization
17 Effect of the zona-free aggregation on the developmental competence of kodkod (Leopardus guigna) embryos generated by interspecies somatic cell nuclear transfer
189 EFFECT OF THE OVARIAN STIMULATION OF ANESTROUS CATS WITH eCG ON MORPHOLOGICAL QUALITY AND GENE EXPRESSION PROFILE OF CUMULUS-OOCYTE COMPLEXES
Live birth of domestic cat by in vitro fertilization using oocytes recovered after mild follicular stimulation with equine chorionic gonadotropin
103 OVARIAN STIMULATION OF ANESTROUS CATS WITH FOLLICLE-STIMULATING HORMONE IMPROVES OOCYTE QUALITY AND DEVELOPMENTAL CAPABILITY AFTER PARTHENOGENETIC ACTIVATION
105 BOVINE DEMI-BLASTOCYSTS ARE ABLE TO EXPAND TO A SIZE SIMILAR TO INTACT BLASTOCYSTS UNTIL AT LEAST DAY 13 OF IN VITRO CULTURE
CELL CYCLE SYNCHRONIZATION ANALYSIS OF SKIN FIBROBLASTS FROM DOMESTIC CAT (FELIS SILVESTRIS CATUS) AND KODKOD (LEOPARDUS GUIGNA)
137 splitting of ivp bovine blastocyst affects morphology and global gene expression of resulting demi-embryos during elongation.
212 INTERSPECIFIC CLONING AND EMBRYO AGGREGATION INFLUENCE THE EXPRESSION OF oct4, nanog, sox2, AND cdx2 IN CHEETAH AND DOMESTIC CAT BLASTOCYSTS
184 isolation and characterization of bovine endometrial stem cells
94 efficient bovine embryo splitting for gene expression and in vivo development studies.

Proyecto (7)

Calcio y fertilidad: interacciones moleculares proteína- proteína determinantes en la fisiología reproductiva humana y animal.
Evaluación del rol de la zona pelúcida como agente regulador de las señales moleculares embrionarias que promueven el reconocimiento materno durante el periodo peri-implantatorio en la gata doméstica
Desarrollo de un sistema no invasivo para la predicción de calidad y el diagnóstico genético pre-implantatorio para embriones bovinos producidos in vitro.
Desarrollo de un sistema de predicción de calidad y de diagnóstico genético pre-implantatorio no invasivo para embriones producidos in vitro en la clínica humana
Desarrollo de soluciones tecnológicas para la conservación, recuperación y producción de recursos genéticos requeridos por el sector bovino de leche
Identification of pluripotency and inflammatory markers in the uterine endometrium of healthy and endometritis diagnosed cows and their role in endometrial function
Analysis of the expression of pluripotence markers in bovine day-7 blastocysts produced in vivo, in vitro and by scnt and its influence on further embryonic development to the elongation stage
39
Daniel Veraguas

Assistant Professor

Ciencias Agrarias

Universidad Católica del Maule

Curicó, Chile

7
FIDEL CASTRO

DIRECTOR LABORATORIO

FACULTAD DE CIENCIAS VETERINARIAS

UNIVERSIDAD DE CONCEPCIÓN

Chillan, Chile

6
Lleretny Rodriguez

Profesor Asociado

Ciencia Animal

UNIVERSIDAD DE CONCEPCIÓN

Chillán, Chile

3
Joel Cabezas

Ph.D. in Veterinary Sciences

Ciencia Animal

Universidad de Concepción

Chillan , Chile

1
Mario Briones

Profesor Asociado

Ciencia Animal

Universidad de Concepción

Chillán, Chile

1
Daniela Rojas

Tecnólogo Médico

Patología y Medicina Preventiva

Universidad de Concepción

Chillán, Chile

1
Paulina Pacha

DOCENTE PLANTA

Medicina Veterinaria

Universidad Santo Tomás

TEMUCO, Chile

1
Constanza Aguilera

Docente investigadora

Universidad San Sebastián

Concepción, Chile

1
Fernando Saravia

Profesor Asociado

Ciencia Animal

UNIVERSIDAD DE CONCEPCIÓN

Chillán, Chile