Man

Roberto Aquiles Zúñiga Olate

Investigador Asociado a Proyectos

Universidad de Chile/ Facultad de Medicina

Santiago, Chile

Líneas de Investigación


Desarrollo de Anticuerpos Terapéuticos para uso en cancer y enfermedades autoinmunes. Desarrollo de promotores de la transcripción para mejorar la expresión y producción de anticuerpos monoclonales recombinantes en células CHO.

Educación

  •  Biologia Molecular, UNIVERSIDAD DE CHILE. Chile, 2003

 

Article (17)

Development of a new promoter to avoid the silencing of genes in the production of recombinant antibodies in chinese hamster ovary cells

Zuniga, Roberto A.; Gutierrez-Gonzalez, Matias; Collazo, Norberto; Hernan Sotelo, Pablo; Ribeiro, Carolina H.; Altamirano, Claudia; Lorenzo, Carmen; Carlos Aguillon, Juan; Carmen Molina, Maria

Article ISI SCOPUS
JOURNAL OF BIOLOGICAL ENGINEERING  (2019)

Optimization of culture conditions for the expression of three different insoluble proteins in Escherichia coli

Gutierrez-Gonzalez, Matias; Farias, Camila; Tello, Samantha; Perez-Etcheverry, Diana; Romero, Alfonso; Zuniga, Roberto; Ribeiro, Carolina H.; Lorenzo-Ferreiro, Carmen; Carmen Molina, Maria

Article ISI SCOPUS
SCIENTIFIC REPORTS  (2019)

Transcription factor engineering in CHO cells for recombinant protein production

Gutierrez-Gonzalez, Matias; Latorre, Yesenia; Zuniga, Roberto; Carlos Aguillon, Juan; Carmen Molina, Maria; Altamirano, Claudia

Article ISI SCOPUS
CRITICAL REVIEWS IN BIOTECHNOLOGY  (2019)

Mild hypothermia upregulates myc and xbp1s expression and improves anti-TNF alpha production in CHO cells

Torres, Mauro; Zúñiga, Roberto; Gutierrez, Matias; Vergara, Mauricio; Collazo, Norberto; Reyes, Juan; Berrios, Julio; Aguillón, Juan Carlos; Molina, María Carmen; Altamirano, Claudia

Article ISI SCOPUS
PLOS ONE  (2018)

STAT3 inhibition by STA21 increases cell surface expression of MICB and the release of soluble MICB by gastric adenocarcinoma cells

Garrido-Tapia, Macarena; Hernández, Carolina J.; Ascui, Gabriel; Kramm, Karina; Morales, Marcela; Gárate, Valentina; Zúñiga, Roberto; Bustamante, Marco; Aguillón, Juan Carlos; Catalán, Diego; Ribeiro, Carolina H.; Molina, María Carmen

Article ISI SCOPUS
IMMUNOBIOLOGY  (2017)

Clinical significance of tumor expression of major histocompatibility complex class I-related chains A and B (MICA/B) in gastric cancer patients

Ribeiro, CH; Kramm, K.; Galvez-Jiron, F; Pola, V.; Bustamante, M; Contreras, HR; Sabag A.; Garrido-Tapia, M; Hernandez, CJ; Zúñiga R.; Collazo, N.; Sotelo, PH; Morales C. ; Mercado L.; Catalan, D; Aguillón JC; Molina, MC

Article ISI SCOPUS
ONCOLOGY REPORTS  (2016)

An efficient method for variable region assembly in the construction of scFv phage display libraries using independent strand amplification

Sotelo, P.H.; Collazo, N.; Zúñiga R.; Gutierrez-Gonzalez M.; Catalan, D; Ribeiro, C.H.; Aguillon, J.C.; Molina, M. C.

Article ISI SCOPUS
mAbs  (2012)

Weak CD4+ T-cell responses to citrullinated vimentin in rheumatoid arthritis patients carrying HLA-DR9 alleles

Catalan, D; Aravena, O; Zúñiga R.; Silva N.; Escobar A.; Sabugo, F; Wurmann, P; Soto L.; González R.; Alfaro, J; Larrondo M.; Cuchacovich M.; Aguillon, J.C.

Article ISI SCOPUS
Rheumatology International  (2012)

Interleukin 10 decreases MICA expression on melanoma cell surface

Serrano A.E.; Menares-Castillo, E; Garrido-Tapia, M; Ribeiro, C.H.; Hernandez, C.J.; Gatica-Andrades, M; Valenzuela-Diaz R; Ziga R.; López, M N.; SALAZAR ONFRAY F; Aguillon, J.C.; Molina, M. C.; Mendoza-Naranjo, A

Article ISI SCOPUS
Immunology and Cell Biology  (2011)

Modulation of established murine collagen-induced arthritis by a single inoculation of short-term lipopolysaccharide-stimulated dendritic cells

Salazar, L; Aravena, O; Abello P; Escobar A.; Contreras-Levicoy, J; Rojas-Colonelli, N; Catalan, D; Aguirre A.; Zúñiga R.; Pesce B,; González C; Cepeda R.; Cuchacovich M.; Molina, MC; SALAZAR ONFRAY F; Delgado M.; Toes, RE; Aguillón JC

Article ISI SCOPUS
ANNALS OF THE RHEUMATIC DISEASES  (2008)

Short-term lipopolysaccharide stimulation indeces differentiation of murine bone marrow-derived dentritic cells into tolerogenic phenotype

Salazar, L; Aravena, O; Contreras-Levicoy, J; Pesce B,; Catalan, D; Zúñiga R.; Iruretagoyena M.; Kalergis, AM; Aguillón JC

Article ISI SCOPUS
European Cytokine Network  (2007)

Yeast system as a model to study Moloney murine leukemia virus integrase: expression, mutagenesis and search for eukaryotic partners

Vera, J; Parissi, V; Garcia, A.; Zúñiga R.; Andreola, ML; Caumont-Sarcos, A; Tarrago-Litvak, L; Leon, O.

Article ISI SCOPUS
JOURNAL OF GENERAL VIROLOGY  (2005)

Expression of the C-terminus of HIV-1 reverse transcriptase p66 and p51 subunits as a single polypeptide with RNase H activity

Zúñiga R.; Sengupta, S; Snyder, C.; Leon, O.; Roth, MJ

Article ISI SCOPUS
PROTEIN ENGINEERING DESIGN SELECTION  (2004)

Lysine directed cross-linking of viral DNA-RNA : DNA hybrid substrate to the isolated RNase H domain of HIV-1 reverse transcriptase

Guaitiao, JP; Zuniga, RA; Roth, MJ; Leon, O.

Article ISI SCOPUS
BIOCHEMISTRY  (2004)

A dispensable peptide from Acidithiobacillus ferrooxidans tryptophanyl-tRNA synthetase affects tRNA binding

Zuniga, R; Salazar, J; Canales, M; Orellana, O

Article ISI
FEBS LETTERS  (2002)

A dual-specific Glu-tRNA(Gln) and Asp-tRNA(Asn) amidotransferase is involved in decoding glutamine and asparagine codons in Acidithiobacillus ferrooxidans

Salazar, JC; Zuniga, R; Raczniak, G; Becker, H; Soll, D; Orellana, O

Article ISI
FEBS LETTERS  (2001)

Conserved amino acids near the carboxy terminus of bacterial tyrosyl-tRNA synthetase are involved in tRNA and Tyr-AMP binding

Salazar, JC; Zuniga, R; Lefimil, C; Soll, D; Orellana, O

Article ISI
FEBS LETTERS  (2001)

Patent (1)

Promoter with a region rich in cytosine-guanine dinucleotides, vectors, cellular lines, method for producing recombinant protein

Aguillon, Juan C.

Patent
 (2018)

18
Roberto Zúñiga

Investigador Asociado a Proyectos

Programa Disciplinario de Inmunologia

Universidad de Chile/ Facultad de Medicina

Santiago, Chile

11
MARIA MOLINA

Director

Instituto de Ciencias Biomédicas

Universidad de Chile

INDEPENDENCIA, Chile

8
JUAN AGUILLON

PROFESSOR

IMMUNOLOGY PROGRAM

UNIVERSIDAD DE CHILE

SANTIAGO, Chile

6
Diego Catalán

Associate Professor

Programa Disciplinario de Inmunología

Universidad de Chile

Santiago, Chile

5
Matias Gutiérrez

Investigador

Programa Disciplinario de Inmunología

Universidad de Chile

Santiago, Chile

4
Norberto Collazo

Business Developer

Center for Sustainable Biotechnology

Fraunhofer Chile Research

Santiago, Chile

3
OCTAVIO ARAVENA

Gestión I+D

I+D+i

Comisión Chilena de Energía Nuclear

Santiago, Chile

3
Claudia Altamirano

Profesor Titular

Facultad de Ingeniería

PONTIFICIA UNIVERSIDAD CATOLICA DE VALPARAISO

Valparaíso, Chile

2
Alejandro Escobar

assistant professor

Institute for Research in Dental Sciences

UNIVERSIDAD DE CHILE

Santiago, Chile

2
Carolina Ribeiro

Assistant Professor

Immunology Program

UNIVERSIDAD DE CHILE - FACULTAD DE MEDICINA

Santiago, Chile

2
Lorena Salazar

Academico

Instituto Cs Biomédicas

Universidad Autónoma de Chile

Santiago, Chile

2
Bárbara Pesce

Profesora Asistente

ICBM

Universidad de Chile

Santiago, Chile

2
Lorena Salazar

Profesora Asistente

Instituto de Ciencias Biomédicas

Universidad Autónoma de Chile

Lampa, Chile

1
Maria Sabugo

Medico Reumatologo

Medicina

UNIVERSIDAD DE CHILE HOSPITAL CLÍNICO

Santiago, Chile

1
Pamela Wurmann

reumatologa

medicina

universidad de chile

santiago, Chile

1
Jorge Vera

Docente de planta

Universidad Santo Tomás

Puerto Montt, Chile

1
Paula Abello

Docente Adjunto

Ciencias Biológicas

Universidad San Sebastián

Santiago, Chile

1
LUIS MERCADO

Profesor Titular

Instituto de Biología

Pontificia Universidad Católica de Valparaíso

Valparaíso, Chile

1
Hector Contreras

Academico

Oncologia Basico Clinica

UNIVERSIDAD DE CHILE, FACULTAD DE MEDICINA, INSTITUTO DE CIENCIAS BIOMÉDICAS, PROGRAMA DE FISIOLOGÍA Y BIOFÍSICA

Santiago, Chile

1
Marcela Gatica

Coordinadora

Decanatura Facultad de Ciencias

Universidad de La Serena

La Serena, Chile

1
Mercedes López

Profesora Asociada

Instituto de Ciencias Biomédicas

Universidad de chile

Santiago, Chile

1
Lilian Soto

Rheumatologist

Medicine

CLINICAL HOSPITAL OF UNIVERSIDAD DE CHILE

Santiago, Chile

1
Rodrigo Valenzuela

Bioquímico

Hematologia

Hospital del Salvador

Santiago, Chile

1
Julio Berrios

Académico Full-time

Facultad de Ingeniería

PONTIFICIA UNIVERSIDAD CATÓLICA DE VALPARAÍSO

Valparaíso, Chile

1
Mirentxu Iruretagoyena

Jefe Seccion Inmunologia

Laboratorio Clinico

Clinica Alemana de Santiago

Santiago, Chile

1
Antonio Serrano

UNIVERSIDAD CATÓLICA DEL NORTE

Antofagasta, Chile

1
Yesenia Latorre

Investigador

Laboratorio cultivo células animales

Pontificia Universidad Católica de Valparaíso

Valparaíso, Chile

1
Felipe Galvez

Estudiante Doctorado

Universidad de La Frontera

Temuco, Chile